MYC (phospho T58) polyclonal antibody

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	-Rabbit (1:1000)

Protocol tips
-Rabbit (1:1000)

Publication protocol

Three commercial anti-phosphopeptide antibodies were compared to the recombinant FHA domains generated in this report. Two were polyclonal antibodies (pAb), pAbαMyc (Abnova, catalog# PAB0541) and pAbαCaMKII (Thermo Scientific, catalog# PA5-35521), and one was a monoclonal antibody (mAb) mAbαERK1-pT202/pY204+ERK2-pT185/pY187 (mAbαERK1/2) (Abcam, catalog# ab136926). As all three are rabbit antibodies, a goat anti-rabbit immunoglobulin G (IgG), conjugated to Horseradish peroxidase (HRP; Abcam, catalog# ab97051), served as the common secondary reagent. Another secondary reagent was the anti-Flag epitope mAb, M2, which was conjugated to HRP (Sigma-Aldrich, catalog# A8592).

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Manufacturer protocol

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