MMP9 Monoclonal Antibody (IA5)

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	-Mouse (1:500)

Protocol tips
-Mouse (1:500)

Publication protocol

Total membranes and nuclear extracts were prepared as described previously, and protein concentrations were determined using the BCA assay (Pierce, USA). The dilution of primary antibodies was as follows: MMP2 (MA1-772, 1:500); MMP9 (MA5-14220, 1:500); MMP13 (MA5-14247, 1:350); FSTL1 (PA5-31113, 1:1500); Rac1 (PA1-091, 1:1000); Cdc42 (PA1-092X, 1:1000); RhoA (MA1-011, 1:1000); TLR4 (MA5-16216, 1:1000); and NF-κB (PA1-186, 1:1000). All primary antibodies were detected with rabbit polyclonal antibodies (Invitrogen). Each membrane was rinsed three times for 15 min and incubated with the secondary horseradish peroxidase-linked antibodies. β-actin was used as the loading reference for data analysis.

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Manufacturer protocol

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