Anti-MMP-13 Antibody, clone VIIIA2

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:200)

Protocol tips
-Mouse (1:200)

Publication protocol

Following extraction using a high salt buffer (pH 7.5), total protein concentration was determined in duplicate (DC Protein Assay, Bio-Rad Laboratories, Hercules, CA). Proteins at 10μg/well were separated on 8% polyacrylamide gels and examined by standard procedures on a Western Blot. Precision plus ProteinTM WesternCTM Standards (Bio-Rad) were used to indicate the molecular weight. Primary antibodies included COL1A1 1:400 (L-19, goat polyclonal, Santa Cruz Biotechnology Inc., Santa Cruz, CA), and COL3A1 1:200 (C-15, goat polyclonal, Santa Cruz). Signal intensity of bands was quantitated via UN-SCAN-IT (version 4.3; Silk Scientific Co, Orem, UT). The blotted membranes were stained with Coomassie Blue and the protein bands were quantified to represent loading control for each well. Protein amounts were expressed as arbitrary units, relative to the loading control and an internal positive control (protein extracts from a human prolapsed vagina) that was loaded in duplicate on each gel.

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Manufacturer protocol

Download the product protocol from Merck Millipore for Anti-MMP-13 Antibody, clone VIIIA2 below.

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