NFκB p50 Antibody (E-10): sc-8414

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:500) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Protocol tips
-Mouse (1:500) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Publication protocol

TNEN buffer were used to lyse cells in the presence of protease and phosphatase inhibitors. SDS-PAGE was used to analyze protein lysates. The specific antibodies used in this study were: NF-κB p65 (Cell Signaling, 4767, Boston, MA), NF-κB p50/52 (Santa Cruz, sc-8414, California), p38α MAPK (Cell Signaling, 9212, Boston, MA), p38β (Cell Signaling, 2339, Boston, MA), p-p38MAPK (T180/182) (Cell Signaling, 9211, Boston, MA), p53 (c12) (Cell Signaling, 2524, Boston, MA), Tak1 (Cell Signaling, 4505, Boston, MA), p-Tak1(T184/187) (Cell Signaling, 4531, Boston, MA), Creb (Upstate, 05767, Boston, MA), p-Creb (Upstate, 6519, Boston, MA), ERK (Cell Signaling, 9107, Boston, MA), p-ERK (Cell Signaling, 9106s, Boston, MA), cFos (Calbiochem, pc38, Darmstadt, Germany), p-cFos (Santa Cruz, sc-81485, California), and β-ACTIN (Santa Cruz, sc-81178, California).

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Manufacturer protocol

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