IL-8 siRNA (h)

siRNA / miRNA gene silencing Human - HUVEC IL-8 Lipid

Experiment
siRNA / miRNA gene silencing Human - HUVEC IL-8 Lipid
Product
IL-8 siRNA (h) from Santa Cruz Biotechnology
Manufacturer
Santa Cruz Biotechnology

Protocol tips

Protocol tips
siRNA concentration-40 nM

Vortex complex mixture (RNAiFect Transfection Reagent and diluted siRNA) for 10 seconds

Incubate the samples for 10–15 min at room temperature (15–25°C) to allow formation of transfection complexes and add to cells.

Incubate further for analysis of cells.

Publication protocol

Knockdown of ATF4, p75NTR and IL-8 was performed as described [19]. An amount of 40 nM ATF4 and IL-8 and 60 nM p75NTR siRNA was transfected into HUVECs by use of RNAiFect Transfection Reagent (Qiagen, Hilden, Germany). The same dose of scramble siRNA was used as a control. Silencing efficiency was verified by qPCR and western blot analysis [20].

Full paper   Login or join for free to view the full paper.

Reviews

IL-8 siRNA (h) from Santa Cruz Biotechnology has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - HUVEC IL-8 Lipid using IL-8 siRNA (h) from Santa Cruz Biotechnology.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for IL-8 siRNA (h) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - HUVEC IL-8 Lipid using IL-8 siRNA (h) from Santa Cruz Biotechnology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms