Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806

Western blotting NF-kB

Experiment
Western blotting NF-kB
Product
Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
-Rabbit (1:1000)

Publication protocol

The following antibodies were used for immunoblotting and full-size images for immunoblottings are presented in Supplementary Fig. 15. Catalogue number and dilutions are indicated in the parentheses. P-IκBα (9246; 1:1,000), IκBα (4812; 1:1,000), P-p105 (4806; 1:1,000), p105 (13586; 1:1,000), P-p65 (3033; 1:1,000), p65 (8242; 1:1,000), P-IKKα/β (2697; 1:1,000), P-JNK (4668; 1:1,000), JNK (5668; 1:1,000), P-ERK (4370; 1:2,000), ERK (4695; 1:2,000), caspase 8 (4790; 1:1,000), cleaved caspase 8 (Asp391) (9496; 1:1,000), caspase 3 (9662; 1:1,000), cleaved caspase 3 (9661; 1:1,000), PARP (9542; 1:1,000), CYLD (8462; 1:1,000), TBK1 (3504; 1:1,000) and Lamin A/C (4777; 1:2,000) were obtained from Cell Signaling. OPTN (clone C-2, sc-166576; 1:1,000), ubiquitin (clone P4D1, sc-8017; 1:1,000), TNFR1 (clone H271, sc-7895; 1:1,000), IKKα/β (clone H-470, sc-7607; 1:1,000), TRADD (sc-46653; 1:1,000) and NEMO (clone FL-419, sc-8330; 1:1,000) were purchased from Santa Cruz Biotechnology. RIP1 (610458; 1:1,000) and FADD (610399; 1:1,000) were from BD Transduction Laboratories. OPTN (A301-829A, Bethyl and Proteintech; 1:1,000), linear ubiquitin (clone LUB9, MABS451, Millipore; 1:1,000), tubulin (CLT9002, Cedarlane; 1:3,000), NEMO (clone EA2-6, K0159-3, MBL; 1:1,000), HOIP (SAB2102031, Sigma-Aldrich; 1:1,000), HOIL-1L (NBP1-88301, Novus Biologicals; 1:1,000) and SHARPIN (14626-1-AP, Proteintech; 1:1,000) were also used.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 below.

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