Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse

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	-Mouse (1:1000)

Protocol tips
-Mouse (1:1000)

Publication protocol

HEK293T cells were transfected with SBP-EGFP-CB1RWT, SBP-EGFP-CB1RΔH9, or empty pcDNA3.1 and left for 24 hr. The cells were serum-starved overnight and then treated with 1 μM ACEA or 0.01% EtOH for 5 min before being lysed in lysis buffer (50 mM Tris-HCl; 150 mM NaCl; 1% CHAPS, ThermoFisher Scientific; protease inhibitors, Roche) with phosphatase inhibitors (Pierce, ThermoFisher Scientific). SDS-PAGE and Western blotting procedures were carried out according to standard protocols.

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