PCNA (PC10) Mouse mAb #2586

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:1000)

Protocol tips
-Mouse (1:1000)

Publication protocol

Protein extraction and cell fractionation were performed as described before [9]. Thirty micrograms of protein from total extracts per sample were adjusted with Laemmli buffer (Sigma) and loaded on acrylamide/bis-acrylamide gels. Gel electrophoresis, transfer to PVDF membrane (Millipore), and signal development with nitro blue tetrazolium/5-bromo-4-chloro-3-indolylphosphate (NBT/BCIP) solution (Molecular Probes) or chemiluminescence were performed as previously described [9]. Alkaline phosphatase-conjugated anti-mouse or anti-rabbit as well as horse radish peroxidase conjugated anti-mouse, anti-rabbit, and anti-sheep secondary antibodies (1:1000 dilution; Cell Signaling) were used.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for PCNA (PC10) Mouse mAb #2586 below.

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