CREB-2 siRNA (h)

siRNA / miRNA gene silencing Human - HUVEC ATF4 Lipid

Experiment
siRNA / miRNA gene silencing Human - HUVEC ATF4 Lipid
Product
CREB-2 siRNA (h) from Santa Cruz Biotechnology
Manufacturer
Santa Cruz Biotechnology

Protocol tips

Protocol tips
p75NTR siRNA concentration-60 nM

Vortex complex mixture (RNAiFect Transfection Reagent and diluted siRNA) for 10 seconds

Incubate the samples for 10–15 min at room temperature (15–25°C) to allow formation of transfection complexes and add to cells.

Incubate further for analysis of cells.

Publication protocol

Knockdown of ATF4, p75NTR and IL-8 was performed as described [19]. An amount of 40 nM ATF4 and IL-8 and 60 nM p75NTR siRNA was transfected into HUVECs by use of RNAiFect Transfection Reagent (Qiagen, Hilden, Germany). The same dose of scramble siRNA was used as a control. Silencing efficiency was verified by qPCR and western blot analysis [20].

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Papers

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for CREB-2 siRNA (h) below.

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