CREB-2 siRNA (h)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	p75NTR siRNA concentration-60 nM Vortex complex mixture (RNAiFect Transfection Reagent and diluted siRNA) for 10 seconds Incubate the samples for 10–15 min at room temperature (15–25°C) to allow formation of transfection complexes and add to cells. Incubate further for analysis of cells.

Protocol tips
p75NTR siRNA concentration-60 nM Vortex complex mixture (RNAiFect Transfection Reagent and diluted siRNA) for 10 seconds Incubate the samples for 10–15 min at room temperature (15–25°C) to allow formation of transfection complexes and add to cells. Incubate further for analysis of cells.

Publication protocol

Knockdown of ATF4, p75NTR and IL-8 was performed as described [19]. An amount of 40 nM ATF4 and IL-8 and 60 nM p75NTR siRNA was transfected into HUVECs by use of RNAiFect Transfection Reagent (Qiagen, Hilden, Germany). The same dose of scramble siRNA was used as a control. Silencing efficiency was verified by qPCR and western blot analysis [20].

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Manufacturer protocol

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