Anti-CARD15/NOD2 antibody (ab36836)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Rabbit (1:500) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Rabbit (1:500) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

RIPA buffer (Sigma-Aldrich) was used to lyse the cells to obtain total protein lysates. Protein concentration was measured using the BCA method (Sigma-Aldrich). The quantified protein (25 μg) was transferred onto polyvinylidene fluoride (PVDF) membranes (Sigma-Aldrich) following SDS-PAGE gel electrophoresis. Then, the membrane was blocked with 5% non-fat dry milk in tri-buffered saline plus Tween (TBS-T) buffer for 2 h at room temperature and incubated with anti-LRPPRC antibody (1:1000, ab205022, Abcam), anti-NOD2 antibody (1:1000, ab36836, Abcam) or anti-GAPDH antibody (Invitrogen, cat. no. PA1-987) at 4°C overnight, followed by Horseradish peroxidase-conjugated (HRP) secondary antibody (1:5000, Abcam, cat. no. ab7090) at room temperature for 1 h.

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Manufacturer protocol

Download the product protocol from Abcam for Anti-CARD15/NOD2 antibody (ab36836) below.

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