Anti-mTOR antibody (ab2732)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

Sixty micrograms of protein samples were used to perform 10% SDS/PAGE electrophoresis. After electrophoresis, proteins were transferred on to a 0.22-µm nitrocellulose membrane using a semi-dry electroblotter. Membranes were incubated with primary antibodies (1:1000) overnight at 4°C, and then incubated with secondary antibody for 1 h at room temperature (1:5000). Concentration of proteins was detected using ECL immunoblotting reagent. Gray values of bands were quantitated using Scion Image software. GAPDH was used as an internal control. Each experiment was repeated for three times independently.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Western blotting mTOR using Anti-mTOR antibody (ab2732) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Anti-mTOR antibody (ab2732) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Western blotting mTOR using Anti-mTOR antibody (ab2732) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.