Recombinant Anti-CDC42 antibody [EPR15620] (ab187643)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Rabbit (1:10000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Rabbit (1:10000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

Total cellular protein was extracted with RIPA buffer (50 mM Tris base, 1.0 mM EDTA, 150 mM NaCl, 0.1% SDS, 1% Triton X-100, 1% sodium deoxycholate, 1 mM PMSF). Protein samples were separated on 10% SDS-PAGE and transferred onto the PVDF membrane (Amersham Biosciences, Amersham, UK) to detect protein expression level. The PVDF membrane was respectively incubated with IQGAP1 antibody (ab133490, Abcam, 1:1000) or CDC antibody (ab187643, Abcam, 1:10000) at 4°C overnight, followed by three 15-min washes in PBS within 0.1% Tween-20. The membranes were then incubated with HRP-conjugated secondary antibodies at 37°C for 60 min. Detection was performed with Western blot reagent ECL (Amersham Biosciences). Membranes were re-probed with mouse anti-β-actin (sc-1616, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) for normalization of signal as a control.

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Manufacturer protocol

Download the product protocol from Abcam for Recombinant Anti-CDC42 antibody [EPR15620] (ab187643) below.

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