Anti-MMP1 antibody (ab137332)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

Skin tissue samples were lysed in radioimmunoprecipitation assay buffer (Beyotime, Shanghai, China) and total protein concentration was measured with a bicinchoninic acid assay kit (Beyotime). Western blotting was performed as previously described [22] using antibodies against the following proteins: MMP-1 (rabbit polyclonal, 1 : 1000, Abcam, Cambridge, UK, cat. number ab137332); TIMP-1 (rabbit polyclonal, 1 : 200, Santa Cruz Biotechnology, Santa Cruz, CA, USA, cat. number 5538); and JNK (rabbit monoclonal, 1 : 1000, cat. number 9252), phospho-JNK (rabbit monoclonal, 1 : 1000, cat. number 4668), p38 mitogen-associated protein kinase (MAPK) (rabbit polyclonal, 1 : 1000, cat. number 9212), and p-p38 (rabbit polyclonal, 1 : 1000, cat. number 9211) (all from Cell Signaling Technology, Danvers, MA, USA). Glyceraldehyde 3-phosphate dehydrogenase (mouse monoclonal, 1 : 20,000, Sigma, St. Louis, MO, USA, cat. number G9295) served as a loading control. Protein band intensity was quantified using Gene Tools image analysis software (Syngene, Cambridge, UK).

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Manufacturer protocol

Download the product protocol from Abcam for Anti-MMP1 antibody (ab137332) below.

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