MMP-1 Antibody (SB12e): sc-58377

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Protocol tips
-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Publication protocol

Western blot analysis utilised the following antibodies: Cell-Signaling Technologies: IGFBP2 (#3922, 1:1000), phospho-AKTser473 (#4060, 1:2000), total AKT (#2920, 1:1000) HDAC1 (#5356, 1:1000) and HDAC2 (#2545, 1:1000), Santa-Cruz: p53 (sc-126, 1:1000), pERK1/2 (sc-7383, 1:1000), ERK2 (sc-154-G, 1:1000), IGF1R (sc-713, 1:1000), INS-R (sc711, 1:1000), Ets2 (sc-351, 1:2000), MMP1 (sc-58377, 1:1000) and HDAC3 (sc-11417, 1:1000), Sigma-aldrich: beta-actin (A2228, 1:10,000) and ADAM17 (SAB3500367, 1:1000) NcoR1 (Bethyl laboratories A301-145A, 1:1000) and NcoR2 (Abcam Ab24551, 1:1000). Human Growth factor array from RayBiotech was conducted according to manufacturer’s protocols and arrays were analysed using densitometry software, Fluorchem SP. Human IGF-1 ELISA was from RayBiotech. CHIP-qPCR experiments were conducted as previously described [66] using 3 x106 cells per immunoprecipitation. Chip antibodies used in this study as stated above, HDAC3 (2μg per CHIP), NcoR1 (2μg per CHIP), NcoR2 (10μL per CHIP) and H3K9Ac (2.5μg per CHIP).

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Manufacturer protocol

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