Recombinant Anti-Paxillin antibody [Y113] (ab32084)

Western blotting Paxillin

Experiment
Western blotting Paxillin
Product
Recombinant Anti-Paxillin antibody [Y113] (ab32084) from Abcam
Manufacturer
Abcam

Protocol tips

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Rabbit (1:1000)
-To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for
5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

Western blotting was conducted as previously described (59). Primary antibodies were used at a dilution of 1:1000, and secondary antibodies (Licor, P/N 925-32210, P/N 925-32211) were used at a dilution of 1:20,000.
Blots were scanned using the Odyssey CLx machine (Licor). For immunoprecipitation, 500 μg of cell lysates were incubated with 1 μg of antibody against phosphotyrosine (PY20, Thermo Scientific) or control rabbit IgG. The presence of phosphocortactin was detected by immunoblot.

Full paper   Login or join for free to view the full paper.

Reviews

Recombinant Anti-Paxillin antibody [Y113] (ab32084) from Abcam has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Western blotting Paxillin using Recombinant Anti-Paxillin antibody [Y113] (ab32084) from Abcam.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Abcam for Recombinant Anti-Paxillin antibody [Y113] (ab32084) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Western blotting Paxillin using Recombinant Anti-Paxillin antibody [Y113] (ab32084) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms