Phospho-FAK (Tyr397) Recombinant Rabbit Monoclonal Antibody (31H5L17)

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	-Rabbit (1:1000)

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-Rabbit (1:1000)

Publication protocol

Cell samples were lysed in RIPA buffer (Thermo Fisher Scientific). The proteins were resolved by SDS-PAGE using gradient gels (4%–20%) and electroblotted onto PVDF membranes (Thermo Fisher Scientific). The membranes were probed with a rabbit monoclonal anti-CD274 antibody (Cell Signaling Technology, clone E1L3N, 1:1,000 dilution), anti-PDCD1LG2 antibody (Cell Signaling Technology, clone E1L3N, 1:1,000 dilution), anti-p53 antibody (Cell Signaling Technology, clone 48818, 1:1,000 dilution), anti–phospho-FAK (Tyr397) antibody (Thermo Fisher Scientific, clone 31H5L17, 1:1,000 dilution), polyclonal anti-FAK antibody (Cell Signaling Technology, 1:1,000 dilution), and then probed with the appropriate second antibody. GAPDH (Cell Signaling Technology, clone 14C10, 1:1,000 dilution) was used as a loading control.

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