Fibronectin Monoclonal Antibody (FBN11)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:1000)

Protocol tips
-Mouse (1:1000)

Publication protocol

Western blotting was performed as described previously [31]. Cell lysates were prepared with RIPA buffer (150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 50 mM Tris-HCl pH 7.5, 2 mM EDTA, 1 mM PMSF, 1 mM DTT, 1 mM Na3VO4, and 5 mM NaF) containing a protease inhibitor cocktail (Calbiochem, EMD Millipore Corp., Billerica, MA, USA). Proteins were separated using 8% SDS-PAGE and were then transferred to polyvinylidene difluoride (PVDF) membranes. The membranes were probed with the indicated antibodies and developed using Immobilon Western Detection Reagents (Millipore, Billerica, MA, USA). The Bio-ID software was used to quantify protein expression (Vilber Lourmat, Eberhardzell, Germany). All experiments were performed in triplicate.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Western blotting Fibronectin using Fibronectin Monoclonal Antibody (FBN11) from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Fibronectin Monoclonal Antibody (FBN11) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Western blotting Fibronectin using Fibronectin Monoclonal Antibody (FBN11) from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.