Laminin beta-2/gamma-1 Monoclonal Antibody (A5)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Rat (1:500)

Protocol tips
-Rat (1:500)

Publication protocol

Brain samples were homogenized in lysis buffer on ice. Equal amount of proteins was loaded and separated in SDS-PAGE and then transferred to PVDF membranes (Millipore). The membranes were probed with anti-laminin γ1 (Thermo, RT-795, 1:500), Claudin-5 (Thermo, 35-2500, 1:500), ZO-1 (Thermo, 61-7300, 1:500), AQP4 (Millipore, AB3594, 1:500), PDGFRβ (Cell Signaling, 3169, 1:500), and anti-actin (BD, 612657) antibodies over night at 4 °C. Next, the membranes were incubated with appropriate secondary antibodies at room temperature for 1 hour. Proteins were visualized by ECL (PerkinElmer, NEL104001EA) and ChemiDoc Imaging System (Bio-Rad). The density of bands was quantified using Quantity One and normalized to actin. The expression levels of these proteins in PKO-h and PKO-nh brains were normalized to that in control brains. Five animals per group were used for quantification.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Western blotting Laminin subunit Beta-2 using Laminin beta-2/gamma-1 Monoclonal Antibody (A5) from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Laminin beta-2/gamma-1 Monoclonal Antibody (A5) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Western blotting Laminin subunit Beta-2 using Laminin beta-2/gamma-1 Monoclonal Antibody (A5) from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.