Anti-Nanog antibody - ChIP Grade (ab21624)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

The whole lysate of GEFs from post-transfection day 1, 6, 9, 12, 15, 18, and 21 was extracted by following the protocol recommended by the protein extraction kit manufacturer. Western blots were performed by following the methods reported [17]. The detail antibody information were provided as below: Oct 4 (Abcam- Cat. No.: ab19857, dilution ratio 1:1000), Sox 2 (Abcam- Cat. No.:ab97959, dilution ratio 1:1000), Klf 4 (Abcam- Cat. No.: ab72543, dilution ratio 1:1000), C-Myc (BD Biosciences- Cat. No.: 551101, dilution ratio 1:1000), Nanog (Abcam- Cat. No.: ab21624, dilution ratio 1:1000), β-actin (Abcam- Cat. No.: ab8226, dilution ratio 1:1000), goat anti-mouse IgM [FITC] labeled (Abcam - Cat. No.: ab8227, dilution ratio 1:1000).

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Manufacturer protocol

Download the product protocol from Abcam for Anti-Nanog antibody - ChIP Grade (ab21624) below.

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