SOX2 Polyclonal Antibody

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	-Rabbit (1:1000)

Protocol tips
-Rabbit (1:1000)

Publication protocol

Six snap-frozen MDLSCC samples from the original cohort of 10 patients included in DAB IHC staining underwent WB as previously described (32), using primary antibodies for NANOG (1:1,000; cat# ab47102, Abcam), OCT4 (1:2,000; cat# ab109183, Abcam), pSTAT3 (1:1,000; cat# 9145, Cell Signaling Technology), SOX2 (1:1,000; cat# PA1-094, Thermo Fisher Scientific), and β-actin (1:1,000; ab8226, Abcam). Two primary antibodies were used for SALL4 (1:1,000, cat# ab57577 and 1:1,000, cat# ab157172, both from Abcam). Secondary antibodies used were goat anti-rabbit horseradish peroxidase (HRP) conjugate (1:10,000; cat# A16110, Thermo Fisher Scientific) or Alexa Fluor® 647 rabbit anti-mouse (1:2,000; cat# A21239, Thermo Fisher Scientific) as appropriate. HRP conjugated secondary antibody detection was achieved using Clarity™ Western ECL substrate (Bio-Rad). Membranes were imaged using a ChemiDoc MP imaging system (Bio-Rad).

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Manufacturer protocol

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