HIF-1α siRNA (r)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate cells for 48-72 h at 37°C.

Protocol tips
Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate cells for 48-72 h at 37°C.

Publication protocol

pcDNA3.1(+)-Mipu1 eukaryotic plasmid and short hairpin RNA (shRNA) of Mipu1 plasmid (pRNA-u6.1-Mipu1) were constructed in our lab as reported previously [17]. For construction of pcDNA3.1(+)-HIF-1α eukaryotic plasmid, the full length open reading frame of rat HIF-1α gene (NM_024359.1) was generated by PCR. The PCR products were cloned into the unique KpnⅠand EcoRⅠ of pcDNA3.1(+) vector as previously described [20]. The siRNA against to HIF-1α (sc-45919) and control siRNA (sc-37007) were purchased from Santa Cruz. Lipofectamine® 2000 and Lipofectamine® RNAiMAX reagents were purchased from Invitrogen

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for HIF-1α siRNA (r) below.

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