Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
--These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
--These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

Assays were performed as described previously.20 WB analysis and IP were carried out with the following antibodies: FRMPD1 (Sigma, St. Louis, MO, USA, #SAB1407148, 1:500/WB); MYC-tag (Cell Signaling Technology Inc., Danvers, MA, USA, #2276, 1 µg/IP, 1:1,000/WB); GFP (Clontech, CA, USA, #JL-8, 1 μg/IP, 1:3,000/WB); GAPDH (Cell Signaling Technology, #5174, 1:1000/WB); MST1 (Cell Signaling Technology, #3682, 1:1,000/WB); p-MST1 (Cell Signaling Technology, #49332, 1:500/WB); LATS1 (Cell Signaling Technology, #9153, 1:1,000/WB); p-LATS1 (Cell Signaling Technology, #8654, 1:500/WB); YAP (Cell Signaling Technology, #4912, 1:1,000/WB); p-YAP (Cell Signaling Technology, #4911, 1:1,000/WB); LaminB1 (Abcam, Cambridge, MA, USA, #ab16048, 1:1,000/IB); Tubulin (Abcam, #ab52866, 1:1,000/IB). The peroxidase-coupled secondary antibodies were purchased from Santa Cruz Biotechnology Inc. (SantaCruz, CA, USA). Target proteins on PVDF membrane (EMD Millipore, Billerica, MA, USA) were visualized using an ECL kit (Thermo Fisher Scientific, Waltham, MA, USA) and images were obtained using the Bio-Rad Imaging System (Bio-Rad, Hercules, CA, USA).

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Manufacturer protocol

Download the product protocol from Abcam for Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) below.

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