Lamin A/C Monoclonal Antibody (mab636)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Stable at 4°C for one year, for longer term store at -20°C	Mouse (1:1000)

Upstream tips
-Stable at 4°C for one year, for longer term store at -20°C
Protocol tips
Mouse (1:1000)

Publication protocol

Western blot analysis was carried out using 40 μg whole cell or cytoplasmic extracts or 20 μg nuclear extracts per lane. Proteins were separated by SDS-PAGE and electroblotted onto nitrocellulose membranes. Blots were immunostained with primary antibodies against CRABP2 (1:1,000; ProteinTech Group, Inc.; Cat. # 10225-1-AP), p53 (1:1,000; Santa Cruz Biotechnology; Cat. # sc-126), RARα (1:500; Santa Cruz Biotechnology; Cat. # sc-551), RARβ (1:500; Santa Cruz Biotechnology; Cat. # sc-552), cyclin E (1:500, BD Pharmingen; Cat. # 554182), CRYAB (1:1,000; Enzo Life Sciences; Cat. # ADI-SPA-222), GFAP (1:500; BD Pharmingen; Cat. # 556330), β-actin (1:100,000; Sigma; Cat. # A3854), α-tubulin (1:10,000; DSHB; Cat. # 12G10), lamin A/C (1:1,000; Thermo Fisher Scientific; Cat. # MA3-1000) and FABP7 (1:1,000) (Godbout et al. 1998). Primary antibodies were detected with horseradish peroxidase-conjugated secondary antibodies and visualized using the ECL detection system (GE healthcare) or the Immobilon Western detection system (Millipore, Billerica, MA).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lamin A/C Monoclonal Antibody (mab636) below.

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