Mouse Monoclonal Antibody to Lamin A/C Cat# MCA-4C4

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	-Mouse (1:10000)

Protocol tips
-Mouse (1:10000)

Publication protocol

Western blots were performed as previously described (Edens and Levy, 2014a blue right-pointing triangle; Jevtic et al., 2015 blue right-pointing triangle) with the following antibodies: rabbit anti-GFP antibody (A-6455; Invitrogen) used at 1:2000, mouse anti-actin antibody (AM1965b; Abgent) used at 1:200, rabbit pan-PKC antibody (sc-10800; Santa Cruz Biotechnology) used at 1:1000, and mouse Ran antibody used as previously described (Levy and Heald, 2010 blue right-pointing triangle). Xenopus embryo or HeLa cell extracts were probed by Western blot using a rabbit antibody that specifically recognizes LB3 (R12-2960; Assay Biotech) at 1:500 or a mouse antibody against lamin A (MCA-4C4; EnCor Biotechnology) at 1:10,000, respectively. We verified that these lamin antibodies were unique for a given lamin type by testing them against recombinant lamin proteins (Jevtic et al., 2015 blue right-pointing triangle). Secondary antibodies included a 1:15,000 dilution of goat anti-rabbit horseradish peroxidase (HRP; ImmunoPure Antibody 31460; Thermo Scientific) and 1:20,000 dilution of IRDye 800CW anti-rabbit (926-32211; Li-Cor) or IRDye 680RD anti-mouse (925-68070; Li-Cor). Detection was performed as previously described for HRP (Edens and Levy, 2014a blue right-pointing triangle) or infrared fluorescence (Jevtic et al., 2015 blue right-pointing triangle). Band quantification was performed with ImageStudio.

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