P-Cadherin (C13F9) Rabbit mAb #2189

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	-Rabbit (1:1000)

Protocol tips
-Rabbit (1:1000)

Publication protocol

Western blot was conducted as described previously (18). The primary antibodies used in this study were monoclonal antibodies against E-cadherin (1:1,000, #3195; Cell Signaling Technology, Irvine, CA, USA), P-cadherin (1:1,000, #2189; Cell Signaling Technology), Zeb1 (1:500, TA802313; OriGene Technologies, Inc, Rockville, MD, USA), Zeb 2 (1:500, TA802113; OriGene Technologies), Snail (1:100, PA5-23472; Thermo Fisher Scientific, Waltham, MA, USA), Slug (1:1,000, #9585; Cell Signaling Technology), Twist (1:100, sc-15393; Santa Cruz Biotechnology, Dallas, TX, USA), fibronectin (1:1,000, MAB1918; R&D Systems, Minneapolis, MN, USA), WNT1 inducible signaling pathway protein 1 (WISP1) (1:300, PA5-29150; Thermo Fisher Scientific) and lipocalin-2 (LCN2) (1:500, PAB9543; Abnova, Taipei, Taiwan). The secondary antibodies (1:5,000) were anti-rabbit (111-035-003; Jackson Immunoresearch, West Grove, PA, USA) or anti-mouse secondary antibodies (Zymed 81-6520; Zymed, Waltham, MA, USA). The blots were visualized using enhanced chemiluminescence (ECL) reagents (WBKLS0500; Millipore, Billerica, MA, USA). Membranes were visualized using the VersaDoc™ Imaging System (Bio-Rad, Hercules, CA, USA) for analysis.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for P-Cadherin (C13F9) Rabbit mAb #2189 below.

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