SRC Polyclonal Antibody

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	-Rabbit (1:1000)

Protocol tips
-Rabbit (1:1000)

Publication protocol

For western blot analysis, cellular proteins were extracted in 40 mM Tris-HCl (pH 7.4) containing 150 mM NaCl and 1% (v/v) Triton X-100, supplemented with a cocktail of protease inhibitors. Equal amounts of protein were resolved on 10% SDS-PAGE gels, then transferred to a PVDF membrane. After blocking with 5% non-fat milk, the membranes were incubated, first with primary antibody at 4°C overnight, then with HRP-conjugated sheep anti-rabbit or anti-mouse IgG secondary antibodies (Vector, Burlingame, CA). After washing, the blots were developed using the Super Enhanced chemiluminescence detection kit (Applygen Technologies Inc., Beijing, China). Protein bands were visualized after exposure of the membrane to Kodak X-ray film. Antibodies against EREG (AF1195, 0.1 μg/mL) was purchased from R&D Systems. EGFR (44796G, used at 1:1000), EGFR [pY1086] (44790G, used at 1:1000), FAK (34Q36, used at 1 μg/mL), FAK [pY576] (44-652G, used at 1 μg/mL), SRC (44-655G, used at 1:1000), SRC [pY418] (44660G, used at 1:1000) were purchased from Invitrogen.

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