ZO-1 Monoclonal Antibody (ZO1-1A12)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:400)

Protocol tips
-Mouse (1:400)

Publication protocol

Samples were separated by electrophoresis and transferred onto a nitro-cellulose membrane. Incubation with primary antibodies was performed overnight (4 °C) using mouse anti-Zona Occludens 1 (2 μg/ml, 1:400; 339100, Invitrogen, Paris, France), mouse anti-Claudin 5 (1 μg/ml, 1:800; 352500, Invitrogen, Paris, France) or mouse anti-Actin (1:10,000; ab6276, Abcam, Paris, France). Secondary antibody was a goat anti-mouse horseradish peroxidase (HRP)-conjugated (1:4000). Each WB band represents n = 5 and n = 6 mice. Similar protocol and identical antibodies were used for whole-brain tissue homogenates (hippocampi and cortices). WB quantification was performed using Fuji (densitometry).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for ZO-1 Monoclonal Antibody (ZO1-1A12) below.

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