Purified Mouse Anti-Human ZO-1 Clone 1/ZO-1 (RUO)

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	-Mouse (1:1000)

Protocol tips
-Mouse (1:1000)

Publication protocol

Cells were treated with TDPN or DMSO in time-dependent and dose-dependent manners. The cells were harvested by scraping and underwent lysis in RIPA buffer. The BCA method (Thermo Scientific) was used for protein concentration determination. The extracts were analyzed by SDS-PAGE followed by Western blotting with appropriate antibodies.
The following antibodies were used for Western blotting: p21 (catalogue no. 2947), AKT (9272), phosphorylated AKT (9271S), ERK (4695), Slug (9585S), and GAPDH (2118) were from Cell Signaling Technology; E-cadherin (610181) and Zo-1 (610966) were from BD Science Transduction; cyclin E (sc-247), cyclin D1 (sc-246), p53 (sc-126), phosphorylated ERK (sc-7383), collagen I (sc-25974), collagen III (sc-28888), and fibronectin (sc-9068) were from Santa Cruz Biotechnology; and MMP-1 (444209) was from Calbiochem. The secondary antibodies used in the Western blotting were anti-mouse (PI-2000; Vector Laboratories) anti-rabbit (PI-1000; Vector Laboratories), and anti-goat (AP-107P; Millipore). The blots were analyzed using the ImageJ software. The relative change in the ratio of the target protein to the DMSO control was determined.

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