IKKβ Antibody (H-4): sc-8014

Western blotting IKK Beta

Experiment
Western blotting IKK Beta
Product
IKKβ Antibody (H-4): sc-8014 from Santa Cruz Biotechnology
Manufacturer
Santa Cruz Biotechnology

Protocol tips

Protocol tips
-Mouse (1:1000)
-Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink
can fluoresce and cause background.

Publication protocol

Serum-starved HPAEpiCs were incubated with TNF-α at 37°C for the various time points. At the end of treatment, the cells were harvested and centrifuged at 45000 × g at 4°C for 1 h to prepare the whole cell lysate, as previously described (Chi et al., 2012). The denatured samples were analyzed by 10% SDS-PAGE gels and transferred to nitrocellulose membrane. The Western blot was performed by incubation membrane with an anti-cPLA2, anti-p47phox, anti-Gαs, anti-TRAF2, anti-ASK1, anti-TNFR1, anti-NIK, anti-IKKα, anti-IKKβ, anti-p65, anti-phospho-serine or anti-GAPDH (1:1000) antibody for 24 h, and then incubated with an anti-mouse horseradish peroxidase antibody (1:2000) for 1 h. ECL reagents and UVP BioSpectrum 500 Imaging System (Upland, CA, USA) were used to detect and capture the immunoreactive bands. The image densitometry of each immunoreactive band was analyzed and quantified by the UN-SCAN-IT gel software (Orem, UT, USA).

Full paper   Login or join for free to view the full paper.

Reviews

IKKβ Antibody (H-4): sc-8014 from Santa Cruz Biotechnology has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Western blotting IKK Beta using IKKβ Antibody (H-4): sc-8014 from Santa Cruz Biotechnology.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for IKKβ Antibody (H-4): sc-8014 below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Western blotting IKK Beta using IKKβ Antibody (H-4): sc-8014 from Santa Cruz Biotechnology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms