GATA-4 Antibody (G-4): sc-25310

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Protocol tips
-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Publication protocol

Cell lysates from a-TC cells were analyzed using mouse anti-Gata4 (1:1000; SC-25310, Santa Cruz), rabbit anti-Gata6 (1:200; SC-9055, Santa Cruz ) and rabbit anti-Gapdh (1:1000; ab9845, Abcam). Quantification of the relative intensity was performed using ImageJ software.

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Manufacturer protocol

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