Anti-GATA4 Antibody

Protocol tips

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	-Rabbit (1:200)

Protocol tips
-Rabbit (1:200)

Publication protocol

Cells were lysed in RIPA buffer (Sigma-Aldrich, St Louis, MO, USA) complete with protease inhibitors (Roche Diagnostics GmbH, Mannheim, Germany). Protein concentration was quantified by Bradford colorimetric assay (Sigma-Aldrich), and 20 μg of proteins were separated using SDS-PAGE, transferred to PDVF membrane and probed with anti-β-actin (Sigma-Aldrich; A5441), anti-Actc (Santa Cruz Biotechnology, Dallas, TX, USA; SC15335), anti-Gata4 (Abcam, Cambridge, UK; AB4132), anti-Myh7 (Santa Cruz Biotechnology; SC12117), anti-Anf (Abcam; AB5490), anti-cTnT (Abcam; AB10214), anti-Desmin (Sigma-Aldrich; D8281), anti-Flk1 (Millipore, Billerica, MA, USA; 07-716), anti-sarcomeric Actc (Sigma-Aldrich; A7811), anti-acetyl histone H4 (Upstate, Lake Placid, NY, USA; 06-598), anti-monomethyl Lys9 H3 (Upstate; 07-450) and anti-trimethyl Lys4 H3 (Upstate; 07-473).

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Manufacturer protocol

Download the product protocol from Merck Millipore for Anti-GATA4 Antibody below.

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