ICAM-1 Recombinant Rabbit Monoclonal Antibody (9H21L19)

Protocol tips

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	-Rabbit (1-2 µg/mL)

Protocol tips
-Rabbit (1-2 µg/mL)

Publication protocol

Western blotting was performed following one-dimensional SDS-PAGE as follows. Protein samples from EXORaji and Raji cells were electroblotted onto Immobilon P membranes (Millipore Corp, Shanghai, China) and incubated with specific antibodies, followed by horseradish peroxidase-conjugated secondary antibodies, and detected using SuperSignal West Pico chemiluminescent substrate (Pierce Perbio Science, Shanghai, China). Antibodies used in this study to confirm the proteins detected by MS were: anti-HSC70 (clone 13D3; Thorma Scientific, Shanghai, China), anti-ICAM-1 (clone 9H21L19; Thorma Scientific, Shanghai, China), and anti-actin (clone 15G5A11/E2, Thorma Scientific, Shanghai, China).

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