Collagen I antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store as concentrated solution. Centrifuge briefly prior to opening vial. Avoid multiple freeze-thaw cycles.	-Rabbit (1:1000)

Upstream tips
-Store as concentrated solution. Centrifuge briefly prior to opening vial. Avoid multiple freeze-thaw cycles.
Protocol tips
-Rabbit (1:1000)

Publication protocol

Cells were lysed with PRO-PREP (iNtRON Biotechnology, Gyeonggi-do, Korea) and incubated on ice for 15 min. The chilled cells were centrifuged at 15 000 × g for 5 min, and the supernatant was then quantified and electrophoresis performed. After the proteins were transferred onto a polyvinylidene fluoride membrane, blocking was then performed using 5% skim milk. Primary antibodics against BMP-7 (orb100464) was purchased from Biorbyt. Antibodies against TGF-β (GTX110630), and collagen I (GTX20292) were purchased from Genetex. Antibody against slug (9585) was purchased from Cell Signaling Technology, and p-Smad 2/3 (sc-11769) was purchased from Santa Cruz. These antibodies were 1000X diluted into the blocking reagent and reacted overnight. The membranes were washed three times with 0.1% Tween 20 in phosphate-buffered saline (PBS; PBST) and incubated with diluted secondary antibodies. All proteins were detected using the Western Lightning Plus ECL reagent.

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Manufacturer protocol

Download the product protocol from GeneTex for Collagen I antibody below.

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