miRNeasy Mini kit

RNA isolation / purification Cells - immortalized OS-RC-2

Experiment
RNA isolation / purification Cells - immortalized OS-RC-2
Product
miRNeasy Mini kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To capture a higher amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step.

Publication protocol

Total RNA was extracted using miRNeasy Mini Kit (Qiagen, Hilden, Germany). For miRNA quantification, 100 ng total RNA was either reverse transcribed directly using stem-loop primers [44], or was polyadenylated with polyA polymerase (NEB, Beverly, MA, USA) then reverse-transcribed with an oligo-dT adapter primer into cDNAs for quantitative real-time PCR [45].

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized OS-RC-2 using miRNeasy Mini kit from Qiagen.

Paper title
Let-7d suppresses growth, metastasis, and tumor macrophage infiltration in renal cell carcinoma by targeting COL3A1 and CCL7
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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Mini kit below.

Download PDF Download manufacturer protocol

Videos

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