Monoclonal Anti-Collagen, Type III antibody produced in mouse

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	-Mouse (1:500)

Protocol tips
-Mouse (1:500)

Publication protocol

The protein expression of IL-1β, MyD88, MMP-1, MMP-9, TLR-2, collagen I, collagen III and actin were assessed in the total extract of SMC or MAC obtained by homogenizing the cells in Laemmli electrophoresis buffer as described [22]. The signals were visualized using SuperSignal West Pico chemiluminescent substrate (Pierce) and quantified by densitometry employing gel analyzer system Luminiscent image analyzer LAS 4000 (Fujifilm) and Image reader LAS 4000 software.

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