Anti-Integrin α2 Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Rabbit (1:1000)

Protocol tips
-Rabbit (1:1000)

Publication protocol

After IRB approval and informed consent, surgical specimens from either BAVM resection of non-embolized nidal tissue (cases, n=6), or temporal lobe samples obtained from the surgical treatment of epilepsy (controls, n=3) were frozen and homogenized in RIPA buffer (G Biosciences). Western blots were performed with specific antibodies to DV (R&D systems, MAB2364), α5 (Millipore, AB1928), α2 (Chemicon International, AB1936), VEGF (Abcam, ab9570), TGFβ (Abcam, ab66043), CD31 (Abcam, ab54211), and β-actin (Abcam, ab13822). Blots were quantified using ImageJ software.

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Manufacturer protocol

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