Anti-SOX9 Antibody AB5535

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Rabbit (1:500)

Protocol tips
-Rabbit (1:500)

Publication protocol

Primary cells and spheres were crushed with RIPA buffer (150 mM NaCl, 0.5% sodium deoxycholate, 0.1% SDS, 1% NP40, 1 mM EDTA and 50 mM Tris pH 8.0), followed by separation with SDS–PAGE. Then samples were transferred to NC membrane and incubated with primary antibody in 5% milk. After washing with TBST three times, membranes were blotted with HRP-conjugated secondary antibodies for visualization.

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Manufacturer protocol

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