Recombinant Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Rabbit (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

Proteins from cell extracts (about 20 μg) were analyzed by Western blotting using mouse anti-GKN1 at 1:500, rabbit anti-Histone H3 (tri methyl K9) at 1:1000, rabbit anti-HDAC1 at 1:1000, mouse anti-SUV39H1 at 1:500, anti-GAPDH at 1:1000 and rabbit anti-Lamin A/C at 1:1000 dilution. Detection was performed using the enhanced chemiluminescence detection kit (SuperSignal West Pico) following manufacturer’s instructions. Western blot band intensity was measured with ImageJ 1.46r software.

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Manufacturer protocol

Download the product protocol from Abcam for Recombinant Anti-HDAC1 antibody [EPR460(2)] (ab109411) below.

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