mirVana™ miRNA Isolation Kit, with phenol

RNA isolation / purification Cells - immortalized CHO-K1

Experiment
RNA isolation / purification Cells - immortalized CHO-K1
Product
mirVana™ miRNA Isolation Kit, with phenol from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For RNA to dissolve better and to yeild high levels, preheat elution buffer at 95C.

- Addition of β-mercaptoethanol in lysis buffer can be skipped because the phenol will do the job of nuclease inactivation.

- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step..
Downstream tips
- Do vortex for 2-plus minutes during Acid-Phenol:Chloroform extraction step. This will facilitate the removal of tightly bound proteins typically associated with RNA.

Publication protocol

Total RNA was extracted from 2×105 to 2×106 CHO cells using the mirVANATM miRNA Isolation Kit (Life Technologies) according to the manufacturer´s protocol. RNA samples were quantified using a Nanophotometer (Implen, Munich, Germany) at OD 260/280 nm.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized CHO-K1 using mirVana™ miRNA Isolation Kit, with phenol from Thermo Fisher Scientific.

Paper title
Stable microRNA expression enhances therapeutic antibody productivity of Chinese hamster ovary cells.
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for mirVana™ miRNA Isolation Kit, with phenol below.

Download PDF Download manufacturer protocol

Videos

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