FOXP3 Rabbit Polyclonal Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Rabbit (1-2 ug/ml)

Protocol tips
-Rabbit (1-2 ug/ml)

Publication protocol

Western blotting and immunoprecipitation assays were performed as per our previous studies [40]. Thirty μg of protein from each sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred onto nitrocellulose membrane filter, and subsequently immunoblotted with anti-human/mouse Foxp3 purified antibody (clone eBio7979, eBioscience, CA, USA), anti-human Foxp3 (TA319911, Origene, Rockville, MD), and anti-human HIF-1α (category number, 610958; clone, 54/HIF-1α; BD Transduction Laboratories, San Jose, CA or GTX127309, Gentex). β-actin protein served as an internal control.

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Manufacturer protocol

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