VDAC Polyclonal Antibody

Protocol tips

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	-Rabbit (1:1000)

Protocol tips
-Rabbit (1:1000)

Publication protocol

A portion of each tissue was aliquoted into separate tubes containing cold isolation buffer (250 mm sucrose, 1 mm EGTA, 10 mm HEPES, 10 mm Tris–HCl pH 7.4) as well as protease and phosphatase inhibitors (Pierce #87786 and #78420, Waltham, MA, USA) at the time of harvest and stored at −80 °C until used for immunoblotting and other bench assays as necessary. Western blotting was performed on the NuPAGE® Bis‐Tris gel system (Life Technologies #WG1403BOX, Carlsbad, CA, USA) according to the manufacturer's protocols with primary antibody concentrations of 1000× and secondary antibody concentrations of 10 000×. The following primary antibodies were used: rabbit anti‐human erythrocyte catalase (Athens Research & Technology #01‐05‐030000, Athens, GA, USA), UQCRC2 (Abcam #ab103616, Cambridge, UK), VDAC (Thermo Scientific #PA1‐954A, Waltham, MA, USA), Lamp2a (Invitrogen #51‐2200, Waltham, MA, USA), mouse anti‐mouse multiubiquitin (MBL #D058‐3, Wooburn, MA, USA), eEF2 (Santa Cruz Biotechnology #sc‐166415, Dallas, TX, USA), LC3II/I (Cell Signaling #4108, Denver, MA, USA), Beclin1 (Cell Signaling #3495, Denver, MA, USA), PGC1a (Abcam #ab54481, Cambridge, UK), COX‐IV (Abcam #ab14744, Cambridge, UK). The following secondary antibodies were used: donkey anti‐rabbit, goat anti‐mouse, and rabbit anti‐goat.

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Manufacturer protocol

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