Anti-NLRP3 antibody (ab214185)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Rabbit (1:500) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Rabbit (1:500) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

Western blot was performed as previously described. Briefly, proteins of the left hemisphere were extracted by homogenizing in RIPA buffer (Santa Cruz Biotechnology, CA, USA). The primary antibodies were from Abcam (MA, USA) used with following dilution: LAMP1 (1:1000, ab24170), CaMKII (1:5000, ab52476), p-CaMKII (1:2000, ab32678), TAK1 (1:1000, ab109536), p-TAK1 (1:1000, ab109404), JNK (1:2000, ab179461), p-JNK (1:2000, ab131499), NLRP3 (1:500, ab214185), and IL-1β (1:500, ab9787). Caspase-1 was from NOVUS (CO, USA, 1:500, NBP1-45433).

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Manufacturer protocol

Download the product protocol from Abcam for Anti-NLRP3 antibody (ab214185) below.

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