NLRP3/NALP3 (human) monoclonal antibody (Nalpy3-b)

Protocol tips

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	-Mouse (1:1000)

Protocol tips
-Mouse (1:1000)

Publication protocol

HEK293T lysates overexpressing the full-length transcript variant of NLRP3 fused to a DDK tag (identical to FLAG, registered trademark of Sigma-Aldrich) and myc tag or transfected with a mock vector were purchased from OriGene (Rockville, MD). Immunoblotting for NLRP3 was performed on ARPE-19 and THP-1 lysates, using the NLRP3- and mock-transfected HEK293T lysates as controls. Blotting for NLRP3 was followed by stripping and reprobing first for α-tubulin and secondly for DDK.

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Manufacturer protocol

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