Anti-Hsp60 antibody [Mab11-13] - Mitochondrial Marker (ab13532)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Mouse (1:10000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Mouse (1:10000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

For western blotting, after electrophoresis and transfer to PVDF membranes (0.2 mm; Millipore, Bedford, MA, USA), HSP60 was detected with monoclonal Ab against HSP60 (ab13532; Abcam, Cambridge, UK) at a dilution of 1:10 000 and corresponding secondary antibody conjugated with HRP (A9917; Sigma-Aldrich) at a dilution of 1:40 000. Antibodies were detected by Immobilon Western Chemiluminescent HRP Substrate (Millipore) and imaged by Image Quant LAS 4000 (GE Healthcare, Waukesha, WI, USA). For coomassie brilliant blue staining, Coomassie Blue Staining R250 Kit (Beyotime Biotechnology) was used, according to the manufacturer's protocol.

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Manufacturer protocol

Download the product protocol from Abcam for Anti-Hsp60 antibody [Mab11-13] - Mitochondrial Marker (ab13532) below.

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