GLUT1 Polyclonal Antibody

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	-Rabbit (1:500)

Protocol tips
-Rabbit (1:500)

Publication protocol

For Western blotting, samples of cells or tissues were separated on SDS-PAGE gels, transferred to PVDF membranes and probed with primary antibodies as follows: Glut1 (1:2000, Thermo, RB-9052-P0), K10 (1:500, Santa Cruz, SC-70907), K14 (1:1000, Santa Cruz, SC-53253), HSP90 (1:1000, CST, 4877), Involucrin (1:1000, Thermo, MA5-11803), p-ACC Ser79 and ACC (1:1000, CST, 3661, 3662), p-S6 Ser240/244 (1:4000, CST, 5364), COX IV (1:2000, CST, 4844), and followed by secondary antibodies conjugated to HRP at a dilution of 1:5000 (Santa Cruz, Donkey anti-Rabbit: SC2077, Donkey anti-mouse: SC2096) and detection by the ECL System (PerkinElmer, NEL104001EA). Protein content was quantitated by the BCA protein assay kit (Thermo Pierce, 23227).

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Manufacturer protocol

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