Bax antibody [N1N2], N-term

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	-Rabbit (1:1000)

Protocol tips
-Rabbit (1:1000)

Publication protocol

For western blot analysis, equal amounts of proteins were fractionated on appropriate percentage of SDS–polyacrylamide gel and transferred onto polyvinylidene difluoride membranes. After the transfer, the membranes were blocked with 5% nonfat dry milk in PBS containing 0.05% Tween 20 and probed with various primary antibodies. After incubation with secondary antibodies, immunoblots were visualized with the enhanced chemiluminescence detection kit (GE Healthcare). Reproducibility was confirmed in three separate experiments and representative data were shown.

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