OxPhos Complex IV subunit IV Monoclonal Antibody (20E8C12)

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	-Mouse (1:500)

Protocol tips
-Mouse (1:500)

Publication protocol

Western blotting analyses were performed as described previously 12, using the following primary antibodies (Target, Manufacturer, Reference, Dilution factor): ATP synthase α, Life Technologies, A21350, 1:1,000; ATP synthase β, Life Technologies, A21351, 1:2,000; COX I (Cytochrome c oxidase, subunit 1), Abcam, Cambridge, UK, http://www.abcam.com, ab14705, 1:2000; COX II (Cytochrome c oxidase, subunit 2), Abcam, ab110258, 1:2000; COX IV (Cytochrome c oxidase, subunit 3), Life Technologies, A21348, 1:500; Histone 3, Cell Signaling Technology, Danvers, MA, https://www.cellsignal.com, #4499, 1:5,000; PGC‐1α (peroxisome proliferator‐activated receptor gamma, coactivator 1 alpha), Santa Cruz Biotechnology, Dallas, TX, https://www.scbt.com, SC‐13067, 1:500; TCF7L2, Cell Signaling Technology, #2569, 1:1,000; ubiquinol‐cytochrome c reductase, complex III subunit VII (UQCRQ), Proteintech, 14975–1‐AP, 1:2,000.

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