DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1.5 × 10^5 cells were platted	- 20 μM DCFDA was treated to the cells

Upstream tips
- 1.5 × 10^5 cells were platted
Protocol tips
- 20 μM DCFDA was treated to the cells

Publication protocol

Cellular Reactive Oxygen Species Detection was performed using a Flow Cytometry based method with a DCFDA-Cellular Reactive Oxygen Species Detection Assay Kit (Abcam, #ab113851). For the in vivo studies, splenocytes were harvested from MRL.lpr mice after treatment with CDDO-me or placebos or dexamethasone. An aliquot of 1.5 × 105 cells was stained with 20 μM DCFDA or antibodies to cell surface markers (B220, CD4 and CD11b). DCF was excited by the 488 nm laser and detected at 535 nm. For the in vitro studies, splenocytes were isolated from 4-month-old MRL.lpr mice and treated with a STAT3 inhibitor (1 μM of Cucurbitacin I, Santa Cruz Biotechnology) or MEK1 inhibitor (50 μM of PD98059, Cell Signaling Technology) for 4 hours. These cells were then stained with 20 μM DCFDA or antibodies to cell surface markers (B220, CD4 and CD11b) for flow cytometric analysis.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - mouse splenocytes using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ROS assay cell type - mouse splenocytes using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.