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Cells were lysed using the Bright-Glo Luciferase Assay System (Promega), and luminescence was measured on a SpectraMax L microplate reader (Molecular Devices) and normalized to protein content by NanoOrange Protein Quantification Kit (Invitrogen) using the manufacturer’s instructions with slight modifications. |
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Protocol tips |
Cells were lysed using the Bright-Glo Luciferase Assay System (Promega), and luminescence was measured on a SpectraMax L microplate reader (Molecular Devices) and normalized to protein content by NanoOrange Protein Quantification Kit (Invitrogen) using the manufacturer’s instructions with slight modifications. |
Publication protocol
Cells were lysed using the Bright-Glo Luciferase Assay System (Promega), and luminescence was measured on a SpectraMax L microplate reader (Molecular Devices) and normalized to protein content by NanoOrange Protein Quantification Kit (Invitrogen) using the manufacturer’s instructions with slight modifications.
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